Sensitive to histone deacetylase inhibitors valproic acid (VPA) and
Rhabdomyosarcoma cells with elevated levels of hTdp1 were more Both HT1080 cells overexpressing hTDP1 and One gene was the histone deacetylase RPD3, for which thereĪre known inhibitors. Genes that when deleted specifically kill cells with high levels of Using SDL screens in yeast, we identified a set of Genetic vulnerability that could be leveraged for selective killing ofĬancer cells through targeting of an unlinked synthetic dosag e lethal (SDL) partner.
Overexpression of dCIN genes represents a Rhabdomyosarcoma lines with elevated human Tdp1 levelsĪlso exhibit CIN that can be partially rescued by siRNA-mediated TATA-box binding factor, cause CIN when overexpressed in humanĬells. Tyrosyl-DNA-phosphdiesterase, and TAF12, an RNA polymerase II Reveals human orthologs known to be recurrently overexpressedĪnd/or amplified in tumors. Genome-wide screens in the budding yeast for yeast genes thatĬause CIN when overexpressed, a phenotype we refer to as dosageĬIN (dCIN), and identified 245 dCIN genes. Overexpression on chromosome instability (CIN), we performed Somatic copy number amplification and gene overexpression areĬommon features of many cancers.
#CELLPROFILER IDENTIFY CHROMOSOMES SERIES#
This contribution is part of the special series of Inaugural Articles by members of the National Academy of Sciences elected in 2016. Michael Smith Laboratories, University of British Columbia, Vancouver, BC, Canada V6T 1Z4 bChild and Family Research Institute, University of BritishĬolumbia, Vancouver, BC, Canada V5Z 4H4 cDepartment of Medical Genetics, University of British Columbia, Vancouver, BC, Canada V6T 1Z3 dBC CancerĪgency, Vancouver, BC, Canada V5Z 4E6 eDepartment of Molecular Genetics, University of Toronto, Toronto, ON, Canada M5S 1A8 fThe Donnelly Centreįor Cellular and Biomolecular Research, University of Toronto, Toronto, ON, Canada M5S 3E1 and gCenter for Cancer Research, National Cancer Institute, Supipi Duffya, Hok Khim Famb,c, Yi Kan Wangd, Erin B. Overexpression screens identify conserved dosageĬhromosome instability genes in yeast and
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